Characteristics of human spermatozoa harvested in culture media with and without serum proteins

Ghofraan A. Ata’Allah, and Noor Azmi Mat Adenan, and Nuguelis Razali, and Siti Khadijah Idris, and Jaffar Ali, and Kannappan Palaniappan, and Rosliza Saad, and Krishnan Kanniah, (2018) Characteristics of human spermatozoa harvested in culture media with and without serum proteins. Jurnal Sains Kesihatan Malaysia, 16 (1). pp. 125-134. ISSN 1675-8161


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This study was aimed to determine the efficiency of synthetic protein-free media in spermatozoa washing, preparation and retention of the activity of washed spermatozoa over short periods in vitro. Normozoospermic semen samples (n = 71) were equally apportioned and washed using synthetic protein-free medium (PFM), minimum essential medium + HSA (MEM) or commercial protein-containing medium (CPC). Washed spermatozoa were cultured in vitro using PFM, MEM or CPC media and held for 24 hrs at 4°C, 15°C, 22°C or 37°C. Spermatozoa activity was evaluated at 0 hr, 4 to 7 hrs and 24 hrs post-wash. The effects of PFM on spermatozoa motility, vitality, membrane integrity and DNA fragmentation level were not significantly different from that of MEM and CPC media at 0 hr, 4 to 7 hrs and 24 hrs post-wash in vitro. Synthetic PFM, MEM and CPC retained spermatozoa activity highest when specimen were held at 22°C and it was significantly higher (p < 0.05) than that at 37°C after 24 hrs incubation in vitro. However, no significant changes (p > 0.05) were noted in spermatozoa DNA fragmentation (SDF) levels when specimen were held at 22°C or 37°C at 4 to 7 hrs and also after 24 hrs post-wash in vitro in all media. The use of synthetic PFM as an alternative to the commercial protein-containing media in human spermatozoa washing and preparation procedure for an efficient and safer (Assisted Reproduction Technology) ART outcome. Spermatozoa activity can be successfully retained at room temperature post-wash over short periods; spermatozoa may lose viability rapidly if held for long hours at 37°C in all media.

Item Type:Article
Keywords:Protein-free medium; In-vitro culture; Temperature; Spermatozoa activity; DNA fragmentation
Journal:Jurnal Sains Kesihatan Malaysia
ID Code:11508
Deposited By: ms aida -
Deposited On:15 Mar 2018 04:21
Last Modified:02 Apr 2018 02:49

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