A non-invasive technique for sex determination of monomorphic birds

Jessey Angat, and Farida Zuraina Md Yusof, (2015) A non-invasive technique for sex determination of monomorphic birds. Malaysian Applied Biology, 44 (4). pp. 27-30. ISSN 0126-8643


Official URL: http://www.mabjournal.com/index.php?option=com_con...


We report on the unambiguous identification of monomorphic bird from a freshly plucked feather in Gunung Ledang, Taman Negara, Johor. Genetic analysis accurately recognizes gender by comparing the intron length between CHDZ and CHDW that is preserved within the avian Z and W sex chromosomes. The species under study were Stripe-throated Bulbul (Pycnonotus finlaysoni), Olive-winged Bulbul (Pycnonotus plumosus), White-throated Kingfisher (Halcyon smyrnensis) and Collared Scops Owl (Otus lettia). PCR amplification using P2/P8 primer pairs produced single band in male (ZZ) and two bands in female (ZW) birds that ranged between 300-400 base pairs in length. The PCR products were confirmed by the preliminary experiment of known sex of both species of polymorphic birds. Thus, this PCR-based test is fast, accurate and applicable in these species. Therefore, it could be used for many conservation studies notably in threatened or rare species.

Item Type:Article
Keywords:CHD genes; Non-invasive; Molecular sexing; Monomorphic bird; P2/P8
Journal:Malaysian Applied Biology Journal
ID Code:11787
Deposited By: ms aida -
Deposited On:26 Jun 2018 01:34
Last Modified:29 Jun 2018 07:56

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