Characterization and screening of lipolytic bacteria from Thai fermented fish

Rungsima Daroonpunt, and Naoto Tanaka, and Masataka Uchino, and Somboon Tanasupawat, (2018) Characterization and screening of lipolytic bacteria from Thai fermented fish. Sains Malaysiana, 47 (1). pp. 91-97. ISSN 0126-6039


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Nine bacterial strains were isolated from Thai fermented fish by the standard dilution technique using JCM no. 377 medium. The rod shaped isolate FN2-3 was identified as Virgibacillus dokdonensis while isolates FN2-3 and FN6-6 as V. halodenitrificans, FN1-13 as Corynebacterium variabile, FN1-10 as Oceanobacillus iheyensis and FN3-7 was Bacillus amyloliquefaciens subsp. plantarum. The coccal isolates, FN6-1 and FN6-7 were Staphylococcus saprophyticus subsp. bovis and FN6-8 was S. saprophyticus subsp. saprophyticus, based on their phenotypic characteristics and 16S rRNA gene sequence analyses at a 99.63-100% sequence similarity. Their lipase activity in complex medium (CM), CM medium with 1% (v/v) Tween 20 or CM medium with 1% (v/v) Tween 80 ranged from 1.12 ± 0.03-3.77 ± 0.04 unit/mL, with the highest lipase activity found with V. dokdonensis FN1-8 cultivated with CM with 1% (v/v) Tween 80.

Item Type:Article
Keywords:Fermented fish; halophilic bacteria; lipase; lipolytic bacteria
Journal:Sains Malaysiana
ID Code:11984
Deposited By: ms aida -
Deposited On:14 Aug 2018 00:29
Last Modified:14 Aug 2018 00:29

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