Effect of gamma irradiation on the physical stability of DPPC liposomes

Liyana Mohd Ali Napia, and Irman Abdul Rahman, and Mohd Yusof Hamzah, and Faizal Mohamed, and Hur Munawar Kabir Mohd, and Intan Syakeela Ahmad Bastamam, and Shamellia Sharin, and Norsyahidah Mohd Hidzir, and Shahidan Radiman, (2018) Effect of gamma irradiation on the physical stability of DPPC liposomes. Sains Malaysiana, 47 (6). pp. 1235-1240. ISSN 0126-6039


Official URL: http://www.ukm.my/jsm/malay_journals/jilid47bil6_2...


Unilamellar liposomes composed of dipalmitoylphosphatidylcholine (DPPC) were prepared by the reverse-phase evaporation method and extrusion through a polycarbonate membrane filter. Liposomes at 0.7 mg/mL lipid concentration in deionized water were exposed to gamma irradiation at a dose in the range 0.5 to 25 kGy. Gamma irradiation of liposomes resulted in the degradation of DPPC lipids into free fatty acids, lysophosphatidylcholine and 1,2-palmitoyl-phosphatidic acid (DPPA). The effect of gamma irradiation towards the physical stability of liposomes was investigated by means of dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential analysis. From the DLS analysis, no significant changes were observed in the hydrodynamic size of liposomes. TEM images indicate that the liposomes surface became smoother and rounder as higher irradiation doses were applied. Zeta potential analysis showed that gamma irradiation of DPPC liposomes at radiation doses as low as 0.5 kGy resulted in a drastic rise in the magnitude of the zeta potential. The results also demonstrate that gamma irradiation of liposomes suspension enhanced the overall stability of liposomes. Hence, it can be concluded that gamma irradiation on DPPC liposomes may potentially produce liposomes with higher stability.

Item Type:Article
Keywords:Dipalmitoylphosphatidylcholine (DPPC); Gamma irradiation; physical stability; Zeta potential
Journal:Sains Malaysiana
ID Code:12128
Deposited By: ms aida -
Deposited On:24 Sep 2018 07:34
Last Modified:28 Sep 2018 22:36

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