Microscopic observation of a root-adhering factor Avin_16040 in Azotobacter vinelandii and Escherichia coli during association with rice root

Liew, Pauline Woan Ying and Jong, Bor Chyan and Najimudin Nazalan, (2017) Microscopic observation of a root-adhering factor Avin_16040 in Azotobacter vinelandii and Escherichia coli during association with rice root. Malaysian Applied Biology, 46 (2). pp. 49-58. ISSN 0126-8643

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Abstract

Microbial adherence to plant root is the initial step in a beneficial plant-microbe interaction. Quantitative RT-PCR analysis deduced the Avin_16040 gene showed upregulated expression when Azotobacter vinelandii was adhered to the rice root. By transforming the full-length Avin_16040 gene into a heterologous host Escherichia coli, the recombinant clones displayed filamentous cell shapes in contrast to the rod-shape of wild type cells. Besides full-length gene insert, some E. coli clones were detected to contain truncated Avin_16040 gene inserts but still shape-shifted to filamentous cells. Further analysis by DNA sequencing revealed the shape-shifting E. coli clones contained 3’-end truncated Avin_16040 gene, while E. coli clones containing the 5’-end truncated Avin_16040 gene remained rod-shaped. The cell surface topographies of A. vinelandii and E. coli cells in the presence and absence of Avin_16040 gene and in association with rice root adherence were analysed using atomic force microscopy.

Item Type:Article
Keywords:Avin_16040; Azotobacter vinelandii; Escherichia coli; AFM
Journal:Malaysian Applied Biology Journal
ID Code:12332
Deposited By: ms aida -
Deposited On:15 Nov 2018 04:09
Last Modified:16 Nov 2018 21:19

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