Transforming growth factor beta 3 induced human adipose-derived stem cells for auricular chondrogenesis

Siti Nurhadis Che Omar, and Goh, Bee See and Muhammad Azhan Ubaidah, and Khairul Anuar Khairoji, and Shamsul Sulaiman, and Lokman Saim, and Chua, Kien-Hui (2018) Transforming growth factor beta 3 induced human adipose-derived stem cells for auricular chondrogenesis. Sains Malaysiana, 47 (10). pp. 2349-2358. ISSN 0126-6039

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Official URL: http://www.ukm.my/jsm/malay_journals/jilid47bil10_...

Abstract

The limitation of self-repair and proliferation capacity of chondrocytes in cartilage reconstruction lead to alternative search of cell source that can improve the auricular regeneration. Human adipose-derived stem cells (HADSC) are an alternative cell source that have unique characteristics to self-renew and differentiate into various tissues making it suitable for cell therapy and tissue engineering. This study aimed to examine the chondrogenic differentiation potential of (HADSC) in monolayer culture by the presence of different transforming growth factor beta’s, TFG-β1, -β2 and -β3. HADSC at passage 3 (1.5 × 105 cell/mL) were cultured in chondrogenic medium containing 5 ng/mL of different transforming growth factor beta’s, TFG-β1, -β2 and -β3 for 7, 14 and 21 days. Data analysis was evaluated based on the growth rate of cells, cells morphological changed, production of collagen type II and glycosaminoglycan sulphate (sGAG). The quantitative RT-PCR was carried out to determine the chondrogenic, fibrogenic and hypertrophic gene expression levels. Differentiation of HADSC into chondrocytes using TFG-β indicates the occurrence of the chondrogenesis process. The best chondrogenic differentiation was observed in HADSC induced by TFG-β3 through the chondrocytes-like cells morphology with cells aggregation and high production of proteoglycan matrices compared to other TGF-βs groups. Additionally, the expression of chondrocytes-specific genes such as Type II collagen, Aggrecan core protein, Elastin and Sox 9 was high. In conclusion, this study has showed that TGF-β3 is the potential growth factor in producing chondrogenic cells for auricular cartilage tissue engineering.

Item Type:Article
Keywords:Chondrocytes; Chondrogenic differentiation; Human adipose-derived stem; Induction; Tgf-β3
Journal:Sains Malaysiana
ID Code:12504
Deposited By: ms aida -
Deposited On:23 Jan 2019 03:54
Last Modified:28 Jan 2019 15:20

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