Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa

Kang, Wern Ni and Azila Adnan, (2019) Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa. Malaysian Applied Biology, 48 (1). pp. 7-14. ISSN 0126-8643


Official URL: http://mabjournal.com/index.php?option=com_content...


Microbial cellulose (MC) is an alternative source of cellulose that can be produced by different types of microorganisms such as Pseudomonas, Komagataeibacter, Sarcina and Azotobacter and they are commonly used in biomedical applications. However, the main issue in MC production is low yield. To enhance MC production, different combinations of the dualsaccharides, such as 25 g/L glucose + 25 g/L galactose, 25 g/L glucose + 25 g/L fructose, 25 g/L glucose + 25 g/L maltose and 25 g/L glucose + 25 g/L lactose were added into culture medium. The fermentation was carried out at 30oC for 5 days in incubator shaker. The combination of glucose and galactose (1:1) gave a higher concentration of MC with 1.23 g/L ± 0.15. However, it was lower than glucose (control) (1.8 g/L ± 0.63). The glucose + galactose concentrations varied between 10 g/ L, 30 g/L, 50 g/L and 70 g/L for the MC production. Therefore, the concentration of 50 g/L is the best for the the MC production. Increasing the concentration of glucose + galactose beyond 50 g/L decreased MC production.

Item Type:Article
Keywords:Microbial cellulose; Fermentation; Pseudomonas aeruginosa; Dual-saccharide
Journal:Malaysian Applied Biology Journal
ID Code:12769
Deposited By: ms aida -
Deposited On:11 Apr 2019 00:55
Last Modified:16 Apr 2019 08:55

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