Mild hyperthermia (39°C) attenuates berberine chloride cytotoxicity against osteosarcoma cells

Mohammed Ali Nashiry, and Alyaa Rajih Al-Khateeb, and Aisha Mohd Din, and Norita Salim, and Aletza Mohd Ismail, and Yeap, Swee Keong and Gabriele Ruth Froemming, (2019) Mild hyperthermia (39°C) attenuates berberine chloride cytotoxicity against osteosarcoma cells. Sains Malaysiana, 48 (5). pp. 1097-1104. ISSN 0126-6039


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Many studies reported that antioxidants and dietary supplements increase tumour progress and reduce survival. Hyperthermia is an adjuvant treatment to sensitise cancer cells for radio- or chemotherapy. The current study was carried out to determine the effects of berberine chloride and hyperthermia on bone cancer cells. MG-63 osteosarcoma cells were treated with hyperthermia (39, 43 and 45°C, respectively) for 1 h. Then, the cells were treated with a low toxic dose of berberine chloride (80 μg/mL). After that, all treated groups were recovered at 37°C for 24 h. Finally, all groups were treated with hyperthermia (39, 43 and 45°C) for the second time (1 h) and were recovered for 3 h at 37°C. Cells exposed to hyperthermia without treatment of berberine chloride were used as hyperthermia control. Cells treated with 80 μg/mL of berberine at 37°C served as berberine control and cells incubated at 37°C were used as an untreated control. All treated groups showed significant apoptosis compared to the control group (p<0.05) except 39°C. On the other hand, mild hyperthermia treatment (39°C) resulted in a reduction of berberine-induced apoptosis (p<0.001). Severe and moderate hyperthermia did not show a significant increase in the rate of apoptosis compared to berberine treated cells. Mild hyperthermia treatment can effectively reduce berberine cytotoxicity and implying negative effects on cancer therapy.

Item Type:Article
Keywords:Apoptosis; Berberine chloride; Hyperthermia; Osteosarcom
Journal:Sains Malaysiana
ID Code:13631
Deposited By: ms aida -
Deposited On:06 Nov 2019 07:22
Last Modified:10 Nov 2019 03:31

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