Human platelet lysate promotes proliferation but fails to maintain chondrogenic markers of chondrocytes

Prana Hardinata Budi Harto, and Muhammad Hanif Mahmud, and Aisya Hanim Othman, and Nurulain Hanani Ngadenin, and Nur Saihah Mohd Azahar, and Muhammad Najib Fathi Hassan, and Nor Hamdan Mohd Yahaya, and Rizal Abdul Rani, and Leong, Chooi Fun and Ng, Min Hwei and Law, Jia Xian (2019) Human platelet lysate promotes proliferation but fails to maintain chondrogenic markers of chondrocytes. Sains Malaysiana, 48 (10). pp. 2169-2176. ISSN 0126-6039

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Official URL: http://www.ukm.my/jsm/malay_journals/jilid48bil10_...

Abstract

Traditionally, foetal bovine serum (FBS) is used as a serum supplement for stem cell expansion in vitro. However, it is associated with xenoimmunisation and the transmission of animal pathogens, which may cause harm to stem cell recipients. As a safer alternative, human platelet lysate (HPL) has been introduced for propagating stem cells. Chondrocytes are expanded in vitro for cartilage repair via autologous chondrocyte implantation (ACI). In this study, we compare the efficacy of HPL prepared from expired platelet concentrates with that of FBS for promoting the proliferation and maintenance of the chondrogenic markers of primary human chondrocytes expanded in vitro. Chondrocytes were cultured in F12:Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 5% HPL, 10% HPL and 10% FBS. The cell morphology, viability and growth rate were examined from passage 1 (P1) to P3. RNA was isolated from P3 cells for quantitative polymerase chain reaction (qPCR) to determine the gene expression level of the chondrogenic, dedifferentiation and hypertrophic markers. HPL promoted chondrocyte proliferation without compromising cell viability. In addition, the chondrocytes cultured with HPL were smaller. However, HPL failed to maintain the chondrogenic markers, except SOX 9 (SRY-box transcription factor 9), which was upregulated, but not significantly. Nonetheless, HPL also suppressed the expression of type X collagen (Col X), a chondrocyte hypertrophic marker. In summary, we demonstrate the benefits of HPL supplementation in human chondrocyte culture, where it enhances cell proliferation and suppresses chondrocyte hypertrophy. In the future, HPL can be used for the large-scale expansion of chondrocytes for ACI.

Item Type:Article
Keywords:Autologous chondrocyte implantation; Cartilage; Chondrocyte; Osteoarthritis; Platelet lysate
Journal:Sains Malaysiana
ID Code:14389
Deposited By: ms aida -
Deposited On:04 Mar 2020 04:25
Last Modified:10 Mar 2020 02:32

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