Anti-viral activity of Phyllanthus niruri against hepatitis C virus

Tutik Sri Wahyuni, and Dzul Azmi, and Adita Ayu Permanasari, and Myrna Adianti, and Tumewu, Lydia and Tri Widiandani, and Utsubo, Chie Aoki and Aty Widyawaruyanti, and Achmad Fuad, and Hotta, Hak (2019) Anti-viral activity of Phyllanthus niruri against hepatitis C virus. Malaysian Applied Biology, 48 (3). pp. 105-111. ISSN 0126-8643


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Hepatitis C virus (HCV) infection is a global problem that causes liver disease and hepatocellular carcinoma. Although the current standard treatment provided a significant improvement on response rate with sustain virology response more than 90%, however, the high cost was remaining limited access to this therapy, resistance emergence and serious side effects which provide the necessities to find the new anti-HCV agents. The current study, we evaluated the ethanol extract of Phyllanthus niruri for its anti-HCV activities. Anti-HCV activity was determined by in vitro culture cells of Huh 7it. Anti-HCV activity of P. niruri extract revealed strong inhibition against HCV with IC50 values of 4.14 µg/mL and yield stronger activity in the entry step of the HCV life cycle. Moreover, the P. niruri extract enhanced anti-HCV activity of simeprevir (NS3 protease inhibitor) with increase the activity up to 4-fold compared to a single treatment of simeprevir. Docking analysis was performed to predict the interaction phyllanthin and hypophyllantin, known compounds of P. niruri against HCV receptor. Both of phyllantin and hypophyllantin were mediated a strong interaction with 4GAG, a protein that involved in entry step of HCV. These results suggested that the ethanol extract of P. niruri may be good candidates for the development of anti-HCV drugs.

Item Type:Article
Keywords:Hepatitis C virus; Antiviral activity; Phyllanthus niruri; Entry step
Journal:Malaysian Applied Biology Journal
ID Code:14693
Deposited By: ms aida -
Deposited On:20 May 2020 05:16
Last Modified:27 May 2020 01:18

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