Effect of purified soluble polysaccharides extracted from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] on 3t3-L1 adipocytes

Decha Sermwittayawong, and Kulwanit Patninan, and Chanawee Jakkawanpitak, and Somruthai Phothiphiphit, and Siwarutt Boonyarattanakalin, and Masashi Inafuku, and Hirosuke Oku, and Kusumarn Noipha, and Nongporn Hutadilok-Towatana, (2020) Effect of purified soluble polysaccharides extracted from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] on 3t3-L1 adipocytes. Sains Malaysiana, 49 (1). pp. 103-112. ISSN 0126-6039


Official URL: http://www.ukm.my/jsm/malay_journals/jilid49bil1_2...


Functional mushroom polysaccharides have been known to the Asian traditional medicine for many years. The anti-obesity activity of mushroom polysaccharides has been illustrated both in vivo and in vitro. However, the effect of pure polysaccharides on isolated fat cells has not been demonstrated. In this study, we used hot water and ammonium oxalate to extract polysaccharides from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] and subsequently purified with enzymatic digestion, solvent extraction and ion-exchange chromatography. One of the fractions, 7S1-1, has the highest yield and purity and contains polysaccharides with the molecular weight around 500 kDa. In addition, Fourier transformed infrared spectroscopy (FTIR) and monosaccharide composition analyses showed that this 7S1-1 sample contains mostly β-glucan and some mannan. We find that the 7S1-1 fraction does not affect the total amount of triglycerides in the 3T3-L1 adipocytes but promotes a release of glycerol of the fat cells, suggesting that the fraction does not inhibit adipogenesis but stimulates lipolysis. Laminarin, a small β-glucan, confers similar results to the 7S1-1 sample, but it could not stimulate lipolysis as well as the 7S1-1 sample. Therefore, these results suggest a potential anti-obesity activity of the mushroom polysaccharides.

Item Type:Article
Keywords:Anti-obesity; Lipolysis; Pleurotus sajor-caju; Polysaccharides; 3T3-L1 adipocytes
Journal:Sains Malaysiana
ID Code:14736
Deposited By: ms aida -
Deposited On:11 Jun 2020 06:05
Last Modified:17 Jun 2020 07:54

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