Optimization of effervescent tablet-assisted dispersive liquid-liquid microextraction with central composite design for preconcentration of stimulant drug

Nurliyana Tazulazhar, and Loh, Saw Hong and Marinah Mohd Ariffin, and Wan Mohd Afiq Wan Mohd Khalik, (2021) Optimization of effervescent tablet-assisted dispersive liquid-liquid microextraction with central composite design for preconcentration of stimulant drug. Sains Malaysiana, 50 (1). pp. 109-121. ISSN 0126-6039

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Official URL: https://www.ukm.my/jsm/malay_journals/jilid50bil1_...

Abstract

The extraction efficiency of stimulant drug, namely caffeine, was investigated using a 23 central composite design. The values of optimum extraction condition were set at 468 µL of 1-dodecanol, 1 piece of effervescent tablet, and 22 °C of extraction temperature. An enrichment factor was calculated as 72 for 80 mL water sample. The run time was conducted in less than 6 min using a non-polar C18 column and an isocratic mobile phase (methanol: water of 40:60 (v/v)) at a controlled flow rate of 1 mL min-1. A good linear response was achieved in the range of 0.01-0.50 µg mL-1 (R2 > 0.998). Detection and quantification limits were calculated at 0.009 and 0.015 µg mL-1, respectively. The average recoveries at two spiking concentration levels were within the range of 75-105% with RSD < 2% (n = 3). Real samples namely beverages which contained caffeine and river water were tested using the proposed method, and the results ranged 0.021-0.56 µg mL-1. The eco-scale score and green analytical procedure index confirmed the greenness profile of the proposed method through a calculated score of 88 and has 6 green criteria, respectively.

Item Type:Article
Keywords:Pharmaceutically active compound; Response surface methodology; Stimulant drug
Journal:Sains Malaysiana
ID Code:16397
Deposited By: ms aida -
Deposited On:07 Apr 2021 08:37
Last Modified:13 Apr 2021 04:08

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