Vortex assisted liquid-liquid microextraction with back extraction of repaglinide, glibenclamide and glimepiride in water samples

Sohaib Jumaah Owaid Luhaib, and Noorfatimah Yahaya, and Alshishani, Anas and Maizatul Najwa Jajuli, and Mazidatulakmam Miskam, (2021) Vortex assisted liquid-liquid microextraction with back extraction of repaglinide, glibenclamide and glimepiride in water samples. Sains Malaysiana, 50 (5). pp. 1297-1307. ISSN 0126-6039

[img]
Preview
PDF
957kB

Official URL: https://www.ukm.my/jsm/malay_journals/jilid50bil5_...

Abstract

A new analytical method based on vortex-assisted liquid-liquid microextraction with back extraction (VALLME-BE) coupled with high performance liquid chromatography was developed for the simultaneous determination of antidiabetic drugs; repaglinide, glibenclamide, and glimepiride in water samples. Chromatographic separation was achieved using C18 column (250 × 4.6 mm × 5 µm) and methanol-phosphate buffer (pH3.7) in the ratio of 70:30 v/v as a mobile phase at a flow rate of 1 mLmin-1. VALLME-BE was performed using 200 μL of n-octane dispersed into the aqueous sample (10 mL) with the aid of vortexing agitation. Then, the analytes were back-extracted from the organic solvent to 0.05 M NaOH (40 µL). Under these conditions, enrichment factor of 155-fold was achieved. The developed VALLME-BE method showed excellent linearity in the range of 30 to 1000 µgL-1 with limit of detection (LOD) of 0.41-1.66 µgL-1 and limit of quantification (LOQ) of 1.38-5.54. 41-1.66 µgL-1. VALLME-BE was applied for the determination of repaglinide, glibenclamide and glimepiride in water samples with the recoveries ranged from 83-109%. The relative standard deviation for inter-day and intra-day precision was less than 9.9%.

Item Type:Article
Keywords:Glibenclamide; Glimepiride; HPLC-UV; Repaglinide and vortex assisted liquid-liquid microextraction with back extraction
Journal:Sains Malaysiana
ID Code:17407
Deposited By: ms aida -
Deposited On:09 Sep 2021 04:03
Last Modified:14 Sep 2021 02:50

Repository Staff Only: item control page