Immunomodulatory properties of wharton’s jelly-derived mesenchymal stem cells from three anatomical segments of umbilical cord

Lim, Jezamine and Sue, Ping Eng and Yeoh, Wei Yen and Low, Yik Wan and Nur Mohd Shafwan Jusoh, and Ain Syahirah Rahmat, and Amirah Shahrani, and Faiq Bahrani Yahya, and Rushda Adiba Abdul Rahman, and Zainul Rashid Mohamad Razi, and Leong, Chooi Fun and Shinsmon Jose, and Min, Hwei Ng (2021) Immunomodulatory properties of wharton’s jelly-derived mesenchymal stem cells from three anatomical segments of umbilical cord. Sains Malaysiana, 50 (6). pp. 1715-1726. ISSN 0126-6039


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Mesenchymal stem cells (MSCs) are multipotent progenitor cells that are reported to be immune-privileged and immuneevasive. MSCs are capable of differentiating into specific cell types for subsequent use in cell-based therapy. They express low levels of human leucocyte antigen (HLA)-ABC and no HLA-DR. Wharton’s jelly-derived MSCs (WJ-MSCs) were also found to express human leukocyte antigen G (HLA-G), which renders them immunosuppressive. This study aimed to determine whether cultured WJ-MSCs retain their immune-privileged and immune-evasive properties after cell differentiation, and whether these properties differ among MSCs derived from different anatomical segments of the umbilical cord. Umbilical cords of healthy pregnant mothers undergoing caesarean section were obtained and grouped by three anatomical segments: fetal, middle, and maternal segments. WJ-MSCs were isolated, culture-expanded, and differentiated into osteogenic cells. Expression of HLA-DR, HLA-ABC, and HLA-G were quantified using flow cytometry. Both undifferentiated and osteodifferentiated WJ-MSCs were subsequently co-cultured with allogeneic peripheral blood mononuclear cells with/without lipopolysaccharide (LPS) stimulation for five days. Lymphocyte proliferation assay was performed using carboxyfluorescein succinimidyl ester (CFSE) as a tracker. Our results showed no significant difference existed in the HLA profiles among WJ-MSCs from different segments and between WJ-MSCs with and without osteogenic differentiation. Mean levels for HLA-G, HLABC, and HLA-DR were 24.82±17.64, 52.50±18.41, and 1.00±1.68%, respectively. Stimulation with LPS and WJ-MSCs increased peripheral blooc mononuclear cells (PBMC) proliferation. However, PBMC proliferation was significantly lower when PBMCs were co-cultured with osteodifferentiated WJ-MSCs (p < .05; with LPS stimulation and p < .001 without LPS stimulation) than when they were co-cultured with undifferentiated WJ-MSCs. These findings suggest that cultured WJ-MSCs stimulate lymphocyte proliferation and are not immune-privileged. Osteodifferentiated WJ-MSCs reduced the immunogenicity of WJ-MSCs, and this reduction in PBMC proliferation was even more pronounced in the presence of LPS (p < .05). In conclusion, cultured WJ-MSCs are not immune-privileged. Osteodifferentiated WJ-MSCs are less immunogenic than undifferentiated WJ-MSCs, in which case hypoimmunogenicity is more profound under LPS-stimulated conditions.

Item Type:Article
Keywords:Immunomodulation; Peripheral blood mononuclear cells; Umbilical cord; Wharton’s jelly mesenchymal stem cell
Journal:Sains Malaysiana
ID Code:17536
Deposited By: ms aida -
Deposited On:25 Oct 2021 03:23
Last Modified:26 Oct 2021 06:19

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