Engineering Lactococcus lactis as a cell factory for the production of limonene

Nurul ‘Aishah Shaili, and Song, Adelene Ai-Lian and Sarah Othman, and Lian, Lionel Aun In and Janna Ong-Abdullah, and Raha Abdul Rahim, (2022) Engineering Lactococcus lactis as a cell factory for the production of limonene. Sains Malaysiana, 51 (1). pp. 95-105. ISSN 0126-6039

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Official URL: https://www.ukm.my/jsm/malay_journals/jilid51bil1_...

Abstract

Limonene is a plant monoterpene which contributes significantly to the scent of most essential oils due to its pleasant fragrance. The compound had been reported to have anti-cancer properties against several types of cancer including colorectal cancer. However, the production of this compound in nature is limited because it is produced as a secondary metabolite. To overcome these challenges, Lactococcus lactis was developed as a heterologous host for the production of limonene. A synthesized limonene synthase (LS) from Mentha spicata (mint) was cloned into L. lactis NZ9000. Western blot analysis using mouse IgG His-Tag monoclonal antibody showed successful LS expression by L. lactis at the size of ~55 kDa. GC-MS analysis results showed that limonene production was optimum after 24 h of induction (~8.0 ppm). Metabolic engineering was attempted to enhance the limonene production by overexpression of lactococcal 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) and mevalonate kinase (mvk) genes in the bacterial host. The recombinant L. lactis carrying pNZ:LSMM plasmid successfully enhanced the limonene production to two-fold (~15.1 ppm) after 24 h of induction. The outcomes of this study show the potential of L. lactis to produce plant proteins and bioactive compounds production, which prospectively leads to an oral delivery system for anti-cancer compounds.

Item Type:Article
Keywords:Isoprenoid; Lactic acid bacteria; Metabolic engineering; Monoterpene
Journal:Sains Malaysiana
ID Code:18350
Deposited By: ms aida -
Deposited On:06 Apr 2022 04:26
Last Modified:11 Apr 2022 06:17

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