Malaysian Tualang honey suppresses the angiogenic events in endothelial cells induced by vascular endothelial growth factor

Ahmad Firdaus Abdul Khalid, and Ng, Chin Theng and Fong, Lai Yen and Tan, Jun Jie and Fahmi Yakop, and Nur Aqilah Kamaruddin, and Muhammad Nazrul Hakim Abdullah, and Yong, Yoke Keong (2022) Malaysian Tualang honey suppresses the angiogenic events in endothelial cells induced by vascular endothelial growth factor. Sains Malaysiana, 51 (3). pp. 815-822. ISSN 0126-6039


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Anti-VEGF therapy has been used as the anti-angiogenic agent in cancer treatment. However, the treatment often couples with severe complications. Complementary natural products that offer similar anti-angiogenic potency with less side effects are sought as alternatives. Study showed that Malaysian Tualang Honey (MTH) can inhibit inflammation-induced vascular hyperpermeability in vitro and in vivo, but the effect on the angiogenesis process remains unclear. Thus, this study aims to determine the anti-angiogenic effects of MTH. Effects of MTH ranging from 0.3% to 0.9% on VEGF-induced human umbilical vein endothelial cells (HUVEC) angiogenesis was determined by proliferation, migration and tube-formation assays. Matrix metalloproteinase-2 (MMP-2) secretion from HUVEC and VEGF production from MCF7 cancer cells in response to MTH were also quantified by using ELISA kits. Suramin, an angio-suppressive agent was used as positive control. MTH significantly suppressed HUVEC proliferation (from 155% proliferation rate to 54%); migration (~ 50% inhibition rate) and tube-formation (69% reduction) induced by VEGF. These findings were explained by the significant reduction (p < 0.05) of VEGF-induced MMP-2 secretion in HUVEC with 39% suppression exhibited by 0.9% of MTH. MTH also significantly (p < 0.05) reduced VEGF secretion (19% reduction compared to control) in MCF7 breast cancer cells. Our findings suggest that the anti-angiogenic effects of MTH mainly targets endothelial cell through inhibition cell proliferation, migration and tube formation capacity, via suppression of MMP-2 secretion by the endothelial cells. However, MTH has less prominent effect on suppressing VEGF secretion by the MCF7 cancer cells.

Item Type:Article
Keywords:Angiogenesis; Human umbilical veins endothelial cells; Matrix metalloproteinases-2; Migration; Proliferation; Tube formation
Journal:Sains Malaysiana
ID Code:19166
Deposited By: ms aida -
Deposited On:27 Jul 2022 03:22
Last Modified:01 Aug 2022 04:19

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