Effect of processing condition on the yield of Oscimum Gratissimum extract

Abstract

Ocimum gratissimum (O. gratissimum) is a medicinal plant which native in Southeast Asia. Containing various compound with medicinal value, it acts as herb and spices in cooking, perfumery and used in traditional preparation. It is reported that O. gratissimum has many phenolic compounds which responsible for many biological activities such as eugenol, limonene, ocimene and rosmarinic acid. Scientific studies regarding O. gratissimum are widely known due to its beneficial therapeutic properties, such as antioxidant, antibacterial, anti-inflammatory and others. However, different extraction techniques approaches will give different effects on extraction yield of bioactive compounds according to the processing condition such as solvent polarity, concentration of solvent, temperature and solvent to sample ratio that applied. This study applied Soxhlet and Ultrasonic-assisted Extraction (UAE) techniques to determine the extraction yield of O. gratissimum. The results indicated Soxhlet techniques requires 2 hours, sample to solvent ratio; 1:10 and temperature at 60oC to obtained highest yield of O. gratissimum extract with percentage yield at 1.723% compared to others processing conditions. Meanwhile, UAE techniques showed highest yield of O. gratissimum extract at sample to solvent ratio at 1:10 with results 20.60% and 60% Methanol as the best solvent for extraction. Throughout analysis, UAE techniques was chosen as the methods of extraction for O. gratissimum with processing conditions that gives high yield of extraction.