In silico analysis of edible bird’s nest proteins as potential precursors for bioactive peptides

Khuzma Din, and Amiza Mat Amin, and Fisal Ahmad, and Amin Ismail, and Adawiyah Suriza Shuib, (2022) In silico analysis of edible bird’s nest proteins as potential precursors for bioactive peptides. Malaysian Applied Biology, 51 (2). pp. 53-62. ISSN 0126-8643


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The present study aimed to perform an in silico evaluation of edible bird’s nest protein as potential precursors of bioactive peptides, as well as to determine whether such peptides can be released by selected proteolytic enzymes. Six edible bird’s nest (EBN) protein sequences from a previous study were chosen as potential precursors to produce bioactive peptides via in silico method using the BIOPEP database. AMCase protein sequences gave the highest number of bioactivities (16 to 18) and nucleobindin-2 protein gave the lowest number of bioactivities (9) among the other protein sequences. It was found that the most potential bioactive peptides from EBN proteins are angiotensin-converting enzyme (ACE) inhibitors and dipeptidyl peptidase-IV (DPP-IV) inhibitors. Furthermore, in silico proteolysis using six selected enzymes was employed to release both dominant bioactivities in EBN proteins, which were ACE and DPP-IV inhibitors. This study shows that a combination of enzymes, chymotrypsin, and papain, produced the highest number of activities for both ACE and DPP-IV inhibitor peptides with the frequency of occurrence of bioactive peptides of 0.0968 and 0.1104, respectively. The toxic prediction tool, ToxinPred, found that all EBN peptides derived by in silico analysis were non-toxic. The current study proposed that EBN can serve as a potential source of bioactive peptides.

Item Type:Article
Keywords:ACE inhibitor; Bioactive peptides; DPP-IV inhibitor; Edible bird’s nest; In silico
Journal:Malaysian Applied Biology Journal
ID Code:20189
Deposited By: ms aida -
Deposited On:17 Oct 2022 02:50
Last Modified:21 Oct 2022 04:11

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