Gamma-tocotrienol alters protein expression of HepG2 cell line

Farahani ARS, and Zakiah J, and Abdul Rahman M, and Karsani SA, and Wan Ngah WZ, (2008) Gamma-tocotrienol alters protein expression of HepG2 cell line. Medicine & Health, 3 (2). pp. 256-262. ISSN 1823-2140


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Gamma-tocotrienol (GTT) has been shown to exhibit significant antitumor activity in a variety of tumor cells. Previous findings have demonstrated that GTT had antiprolifera-tive effects on a liver cancer cell line (HepG2) with an IC50 value of 170μM. In this study, two dimensional gel electrophoresis (2DE) was used to determine changes in protein expression in HepG2 cell line following treatment with GTT. The ultimate aim is to identify the possible molecular mechanisms involved in GTT antitumor activity. This study is focused on obtaining a 2DE protein profile for HepG2 cell line with and without GTT treatment. In the preliminary analysis of the resulting 2DE profiles, 18 protein spots were found to be differentially expressed in cells treated with GTT. This observa-tion is confirmed by extending the analysis to a larger sample size. By studying the effects of GTT treatment on differential protein expression in HepG2 cells the underly-ing mechanisms involved in the antitumor activity of GTT may be elucidated

Item Type:Article
Keywords:Proteomics; two dimensional gel electrophoresis (2DE); Gamma-tocotrie-nol (GTT); HepG2 cells
Journal:Medicine & Health
ID Code:2032
Deposited By: mr Mustaffa Abu Bakar
Deposited On:22 Jun 2011 07:22
Last Modified:14 Dec 2016 06:30

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