Ginger extract (Zingiber officinale Roscoe) triggers apoptosis in hepatocarcinogenesis induced rats

Yasmin Anum MY, and Shahriza ZA, and Looi ML, and Shafina Hanim MH, and Harlianshah H, and Noor Aini AH, and Suzana M, and Wan Zurinah WN, (2008) Ginger extract (Zingiber officinale Roscoe) triggers apoptosis in hepatocarcinogenesis induced rats. Medicine & Health, 3 (2). pp. 263-274. ISSN 1823-2140


Official URL:


Ginger extract has been reported previously by our group to exhibit anticancer and an-tioxidant effects by reducing tumour burden and lipid peroxidation respectively in he-patocarcinogenesis induced rats. The current study examined the expression of pro-apoptotic protein caspase-8 and anti-apoptotic protein Bcl-2 in hepatocarcinogenesis treated rats. Thirty normal male Wistar rats were divided into 5 groups based on the diet given: i) control (normal rat chow), ii) olive oil, iii) ginger extract (100mg/kg body weight), iv) choline deficient diet + ethionine, CDE (to induce liver cancer) and v) CDE+ ginger extract. Rats were killed at week 8, and liver tissues were excised for immuno-histochemical study to identify pro-apoptotic and anti-apoptotic proteins, caspase-8 and Bcl-2. The observation on H&E staining confirmed the CDE diet induced liver can-cer as indicated by the presence of numerous oval cells. Identification of Bcl-2 expres-sion showed that 91.6% (11/12) of the samples from the CDE group revealed positive staining while treatment with ginger extract however inhibited the expression with only 8.4% (1/12) samples showing positive staining for Bcl-2. As for caspase-8 protein, 41.7% (5/12) of the samples from CDE group showed positive staining, which in-creased to 100% (12/12) with ginger extract treatment. Our findings suggest that gin-ger extract has an anticancer effect by inducing apoptosis in liver cancer cells via up-regulation of the expression of pro-apoptotic protein, caspase-8 and down-regulation of the expression of anti-apoptotic protein Bcl-2

Item Type:Article
Keywords:hepatocarcinogenesis-induced rats; ginger extract; apoptosis; caspase-8; Bcl-2
Journal:Medicine & Health
ID Code:2033
Deposited By: mr Mustaffa Abu Bakar
Deposited On:22 Jun 2011 07:48
Last Modified:14 Dec 2016 06:30

Repository Staff Only: item control page