Monosaccharide profile and antioxidant activities of sulphated polysaccharide extracted from Indonesian brown seaweed (Sargassum sp.)

Lim, Seng Joe and Sharifah Habibah Syed Khalafu, and Böhmdorfer, Stefan and Rosenau, Thomas and Mohamad Yusof Maskat, and Noor Soffalina Sofian Seng, and Noorul Syuhada Mohd Razali, and Wan Aida Wan Mustapha, (2022) Monosaccharide profile and antioxidant activities of sulphated polysaccharide extracted from Indonesian brown seaweed (Sargassum sp.). Sains Malaysiana, 51 (10). pp. 3195-3202. ISSN 0126-6039

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Official URL: https://www.ukm.my/jsm/malay_journals/jilid51bil10...

Abstract

This research was conducted to determine the monosaccharide profile and antioxidant activities of the sulphated polysaccharide fucoidan, extracted from Sargassum sp. harvested in Batam, Indonesia. Crude fucoidan (Fsar) was extracted using low pH (pH 2) acid method, obtaining a yield of 5.5% (w/w), and the chemical properties of Fsar were subsequently compared to commercial fucoidan (Fcomm), obtained from Japan. Sulphate contents were determined using spectrophotometric analysis, where Fsar (5.21±0.35%) and Fcomm (8.80±0.19%) differed significantly (p<0.05). Monosaccharide profiling was performed using methanolysis and gas chromatography-flame ionisation detector (GC-FID). Fsar contained fucose (138.34 µg/g), 4-O-methyl-glucuronic acid (105.39 µg/g), galactose (70.49 µg/g) and glucuronic acid (30.60 µg/g) while Fcomm contained fucose (520 µg/g) and glucuronic acid (62.93 µg/g) as its main composition. Antioxidant activities were analysed through 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion (SOA) and hydroxyl radical (·OH) scavenging activities. DPPH scavenging activity of Fsar (40.67±4.0%) was significantly higher (p<0.05) than Fcomm (10.11±1.0%), while SOA and ·OH scavenging activities showed no significant difference (p>0.05) between Fsar and Fcomm. Fsar showed significant differences (p<0.05) in chemical properties and DPPH scavenging activities compared to that of Fcomm. Fucoidan from Southeast Asia waters has the potential for application as functional ingredients.

Item Type:Article
Keywords:Brown seaweed; DPPH; Fucoidan; Monosaccharide profiling; Sulphated polysaccharides
Journal:Sains Malaysiana
ID Code:20865
Deposited By: ms aida -
Deposited On:20 Dec 2022 00:59
Last Modified:22 Dec 2022 08:45

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