Cytotoxic effect of dillapiole on human breast cancer MCF-7 cells

Indah Mohd Amin, and Nur Batrisyia Ruslan, and Zulinda Ayu Zulkipli, and Nurul Aili Zakaria, and Mohd Taufiq Mat Jalil, and Farizan Aris, (2022) Cytotoxic effect of dillapiole on human breast cancer MCF-7 cells. Malaysian Applied Biology, 51 (4). pp. 29-35. ISSN 0126-8643


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Plant secondary metabolites and their derivatives play a significant role in anticancer drug therapy since they are effective against specific characteristics while reducing side effects. Dillapiole is a phenylpropanoid that holds several bioactivities like anti-fungal, anti-inflammatory, anti-leishmanial, and anti-cancer. This study aims to investigate the possible cytotoxic effect of dillapiole on human breast cancer, MCF-7 cells. Cells were cultured in complete RPMI media and incubated at standard culture conditions. After the cells reached 80% confluency, cells were treated with various concentrations (ranging from 0 μM to 150 μM) of dillapiole and tamoxifen as a positive control. Cells were later incubated at 48 and 72 h. Using WST-1 assay, the cytotoxic effect was determined for both incubation times. Results show tamoxifen inhibited the MCF-7 cells with the IC50 at 75.9 μM and 39.8 μM for 48 and 72 h respectively. Parallel with the positive control results, there was a significant cytotoxic effect of dillapiole against MCF-7 cells at 48- and 72-hr incubation with the IC50 at 92.1 μM and 63.1 μM respectively. Based on these results, dillapiole was cytotoxic against MCF-7 cells and its cytotoxic activity was both in a time and dose-dependent manner (<0.05). The morphological analysis supported the WST-1 assay. Our preliminary finding is in agreement with other previous studies, suggesting that dillapiole appears to be a promising anti-cancer agent and opens a wider possibility of downstream analysis on its underlying cytotoxicity mechanism.

Item Type:Article
Keywords:Anti-cancer; Cytotoxic; Dillapiole; MCF-7 cells; and WST-1 assay
Journal:Malaysian Applied Biology Journal
ID Code:21415
Deposited By: Siti Zarenah Jasin
Deposited On:03 Apr 2023 04:56
Last Modified:03 Apr 2023 04:56

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