Expression of BCL-2 and bax genes induced by an ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in breast cancer cell

Hanif, Monica Mulnia and Tjong, Djong Hon and Syaifullah, and Santoso, Putra and Efrizal, and Roesma, Dewi Imelda (2022) Expression of BCL-2 and bax genes induced by an ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in breast cancer cell. Malaysian Applied Biology, 51 (3). pp. 63-69. ISSN 0126-8643

[img]
Preview
PDF
581kB

Official URL: https://jms.mabjournal.com/index.php/mab/index

Abstract

It is known that the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) has the potential for chemoprevention in MCF-7 cells. However, the expression of Bcl-2 and Bax genes was associated with cancer. Therefore, this study aimed to analyze the Bcl-2 and Bax gene expression induced by the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in MCF-7. The method in this study was an in vitro experiment using MCF-7 control cells and MCF-7 cells induced by the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus), and Real-time PCR was used to examine the expression of Bcl-2 and Bax genes. The result showed that the expression of the Bcl-2 gene had an insignificant decrease (p>0.05) by 15%, and the expression of the Bax gene had a significant increase (p<0.05) by 489% compared to the control. These results support that ovarian extract Singkarak Lake Pufferfish (Tetraodon leiurus) can be alternative cancer chemoprevention.

Item Type:Article
Keywords:Cancer; Chemoprevention; Jabuih fish; Lake Singkarak; MCF-7 cell line
Journal:Malaysian Applied Biology Journal
ID Code:21878
Deposited By: Siti Zarenah Jasin
Deposited On:11 Jul 2023 08:17
Last Modified:11 Jul 2023 08:17

Repository Staff Only: item control page