Hanif, Monica Mulnia and Tjong, Djong Hon and Syaifullah, and Santoso, Putra and Efrizal, and Roesma, Dewi Imelda (2022) Expression of BCL-2 and bax genes induced by an ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in breast cancer cell. Malaysian Applied Biology, 51 (3). pp. 63-69. ISSN 0126-8643
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Abstract
It is known that the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) has the potential for chemoprevention in MCF-7 cells. However, the expression of Bcl-2 and Bax genes was associated with cancer. Therefore, this study aimed to analyze the Bcl-2 and Bax gene expression induced by the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in MCF-7. The method in this study was an in vitro experiment using MCF-7 control cells and MCF-7 cells induced by the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus), and Real-time PCR was used to examine the expression of Bcl-2 and Bax genes. The result showed that the expression of the Bcl-2 gene had an insignificant decrease (p>0.05) by 15%, and the expression of the Bax gene had a significant increase (p<0.05) by 489% compared to the control. These results support that ovarian extract Singkarak Lake Pufferfish (Tetraodon leiurus) can be alternative cancer chemoprevention.
| Item Type: | Article |
|---|---|
| Keywords: | Cancer; Chemoprevention; Jabuih fish; Lake Singkarak; MCF-7 cell line |
| Journal: | Malaysian Applied Biology Journal |
| ID Code: | 21878 |
| Deposited By: | Siti Zarenah Jasin |
| Deposited On: | 11 Jul 2023 08:17 |
| Last Modified: | 11 Jul 2023 08:17 |
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