Probiotics potential of lactic acid bacteria from horse milk in Tambora, West Nusa Tenggara, Indonesia

Sulistiani, and Setiarto, R. Haryo Bimo and Dewi, Fera R. and Yusuf, Dandy and Fathoni, Ahmad and Handayani, Rini and Anggadhania, Lutfi and Anshory, Lutfi and Setianingrum, Ninu and Khusniati, Tatik (2023) Probiotics potential of lactic acid bacteria from horse milk in Tambora, West Nusa Tenggara, Indonesia. Sains Malaysiana, 52 (11). pp. 3121-3134. ISSN 0126-6039


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This study explores the probiotic potential of lactic acid bacteria (LAB) in horse milk from Tambora, West Nusa Tenggara by examining its functional and probiotic attributes, including bile salt tolerance, low pH, simulated gastric juice (SGJ), simulated intestinal juice (SIJ), antibacterial activity, as well as bile salt hydrolase (BSH) genes. Genotyping of LAB was performed using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) analysis and then identified based on the 16S rRNA gene. A total of 25 LAB isolates showed the ability to grow at low pH, tolerant to bile, and survived under SIJ and SGJ conditions. The BSH gene was confirmed in three isolates, namely: SK1-28, SK2-30, and SK2-34. Results of RFLP-PCR analysis showed that the LAB isolates were grouped into three groups based on the number and molecular weight of the differences DNA fragments. The 16S rRNA analysis showed that the first two groups were Lacticaseibacillus rhamnosus, whereas the third group was Lactiplantibacillus plantarum. In addition, all Lacticaseibacillus rhamnosus isolates in group I showed the ability to grow at pH 9.0, but not group II. It can be concluded that Lacticaseibacillus rhamnosus and Lactiplantibacillus plantarum can be used as the indigenous probiotic bacteria source from Indonesia.

Item Type:Article
Keywords:Horse’s milk; Lactic acid bacteria (LAB); Lacticaseibacillus rhamnosus; Lactiplantibacillus plantarum; Probiotics
Journal:Sains Malaysiana
ID Code:23244
Deposited By: Siti Zarenah Jasin
Deposited On:21 Mar 2024 06:39
Last Modified:21 Mar 2024 06:39

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