Development of a fast immunosorbent assay for site-screening dioxin contamination in Vietnam

Dang, Phuong Nam and Nguyen, Van Hoang and Pham, Kien Cuong and Nguyen, Thi Nhung and To, Lan Anh and Le, Duy Khanh and Nghiem, Xuan Truong and Nguyen, Khanh Hoang Viet (2023) Development of a fast immunosorbent assay for site-screening dioxin contamination in Vietnam. Sains Malaysiana, 52 (12). pp. 3449-3461. ISSN 0126-6039

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Official URL: https://www.ukm.my/jsm/english_journals/vol52num12...

Abstract

Dioxins are a group of chemical compounds that cause environmental pollution and many harmful effects on human health. High-Resolution Gas Chromatography/High-Resolution Mass Spectrometry (HRGC/HRMS) is the standard method for determining dioxin concentrations in soil samples and provides the most accurate results. However, this method is time-consuming, costly, and requires modern equipment. Currently, competitive ELISA is a reliable method used for dioxin detection analysis, offering fast implementation time and low cost. Vietnam is a global hotspot for dioxin contamination, with a high number of dioxin samples for analysis. Therefore, it is essential to optimize this reliable, fast, and low-cost ELISA method for it to be applicable and replace the expensive and complex HRGC/HRMS method currently in use in Vietnam. This study presented optimized conditions for ELISA method using commercial antibodies to detect dioxin. The optimal dilution for the anti-dioxin antibody and the conjugated antibody is 1:2000 and 1:1000, respectively. The reconstitution buffer consists of 50% DMSO/H2 O, with the addition of 0.05% Triton X-100. The incubation time for anti-dioxin antibody incubated with dioxin is 60 min, while the incubation time for Horseradish Peroxidase (HRP) conjugated polyclonal antibody incubated with 3,3’,5,5’-Tetramethylbenzidine (TMB) substrate is 10 min. The quenching time for the enzyme-substrate reaction is 5 min. The half-maximal inhibitory concentration (IC50) of this method is 8500 pg/well and the limit of detection (LOD) is 2.02 pg/well. Although there is a difference between the analytical results of the two methods, the well-correlated results demonstrate the potential of the ELISA method for detecting and screening dioxin contamination before performing confirmatory analysis with HRGC/HRMS. These results serve as the basis for the development of a rapid dioxin detection kit, providing a new and efficient method for detecting and screening dioxin contamination in Vietnam.

Item Type:Article
Keywords:Dioxin; ELISA; Fast detection; HRGC/HRMS
Journal:Sains Malaysiana
ID Code:23357
Deposited By: Siti Zarenah Jasin
Deposited On:17 Apr 2024 06:59
Last Modified:17 Apr 2024 06:59

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