An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds

Fatima Musbah Abbas, and ElBushra ElSheikh ElNur, and Normah Mohd Noor Eisa Algaali, and Zainon Mohd Ali, and Roohaida Othman, (2011) An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds. Sains Malaysiana, 40 (4). pp. 339-343. ISSN 0126-6039


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The aim of this study was to establish an effective protocol for callus induction from the seed explants of Solanum dubium and to investigate the callus extract ability in milk clotting activity. The effects of growth regulator, basal media strength and sucrose were studied using different concentrations (0.5, 1.0, 1.5, and 2.0 mg/L) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), naphthalene acetic acid (NAA) and 2,4-dichlorophenoxy-acetic acid (2,4-D) alone or in combination with 0.5 mg/L of 6-benzylamino purine (BAP). For milk clotting activity, about 50 or 100 μL extracts of seed callus was mixed with 2 mL 50% milk held at 55°C for 5 and 10 min until milk clotting occurred. The results showed that NAA alone or in combination with BAP gave a higher callusing percentage (80 to100%) compared to the other plant growth regulators at the same concentrations. When an auxin was supplied in combination with BAP, a significant increase in callusing percentage or degree of callusing was observed. The time required for callus to be developed was shortened and the quality of the induced callus improved. An increase in callus growth in low sucrose (10 g/L) concentration was found be comparable (88%) to high sucrose concentration (30 g/L; 60%). Crude extracts obtained from S. dubium callus were shown to exhibit milk clotting activity.

Item Type:Article
Keywords:Auxin; callus; Gubbien; milk clotting activity; Solanum dubium
Journal:Sains Malaysiana
ID Code:2394
Deposited By: Mr Azam
Deposited On:01 Aug 2011 08:17
Last Modified:14 Dec 2016 06:31

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