Kamarul Rahim Kamarudin, and Aisyah Mohamed Rehan, and Ridzwan Hashim, and Gires Usup, and Hajar Fauzan Ahmad, and Mohd Hanafi Anua, and Mohd Yaman Idris, (2011) Molecular phylogeny of holothuria (mertensiothuria) leucospilota (Brandt, 1835) as inferred from cytochrome c. oxidase I mitochondrial DNA gene sequences. Sains Malaysiana, 40 (2). pp. 125-133. ISSN 0126-6039
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Official URL: http://www.ukm.my/jsm/
Abstract
Holothuria (Mertensiothuria) leucospilota (Brandt 1835), white threads fish or locally known as bat puntil is currently considered as the most abundant sea cucumber species in Malaysia. This study aimed to generate the genetic profile of H. leucospilota from Malaysia and then to determine the phylogenetic relationship between H. leucospilota and other members of genus Holothuria using partial sequences of cytochrome c oxidase I (COI) mitochondrial DNA (mtDNA) gene. In this study, specimens of H. leucospilota were collected from Intan Besar Island, Langkawi, Kedah Darul Aman in the west coast of Peninsular Malaysia. Three main methods namely neighbour joining, maximum parsimony and maximum likelihood were used for the phylogenetic tree reconstruction. Tree topologies showed that H. leucospilota has its own monophyletic clade clearly distinct from the other species. The pairwise genetic distance calculated further supported these findings. In addition, the results also should that the COI mtDNA gene is capable to unravel the phylogenetic relationship of H. leucospilota.
Item Type: | Article |
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Keywords: | Holothuria leucospilota; molecular systematics; partial sequences of cytochrome oxidase I gene; phylogeny tree |
Journal: | Sains Malaysiana |
ID Code: | 2462 |
Deposited By: | Mr Azam |
Deposited On: | 02 Aug 2011 08:07 |
Last Modified: | 14 Dec 2016 06:31 |
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