Ability of acanthamoeba cyst to excyst at different temperatures

Nurul Fariza Rossle, and Mohamed Kamel Abd Ghani, and Anisah Nordin, and Yusof Suboh, and Noraina Ab Rahim, (2011) Ability of acanthamoeba cyst to excyst at different temperatures. Jurnal Sains Kesihatan Malaysia, 9 (1). pp. 41-43. ISSN 1675-8161

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This study was carried out to observe thermotolerance ability of Acanthamoeba spp. A total of 32 Acanthamoeba spp. isolates obtained from water taps, sinks, swimming pools and sea water were used. Trophozoites of Acanthamoeba spp. were inoculated onto non-nutrient agar (NNA) seeded with heat-killed Escherichia coli using aseptic technique and incubated for 14 days at 30°C to obtain the cyst. The cysts were subcultured onto new agar plates for thermotolerance test at 37°C and 42°C. The plates were observed until 96 hours after incubation for excystation of Acanthamoeba before being declared negative. Overall, 81.25% of samples were able to excyst at 37°C while 37.5% were able to excyst at 42°C. Thermotolerant Acanthamoeba is associated with high pathogenicity potential.

Item Type:Article
Keywords:Thermotolerance; Acanthamoeba cyst; Excystation
Journal:Jurnal Sains Kesihatan Malaysia
ID Code:3607
Deposited By: Ms Nur Asyikin Lut Ahmad
Deposited On:01 Mar 2012 03:23
Last Modified:14 Dec 2016 06:34

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