Effect of pH and agitation on growth of bacillus subtilis

Noorhisham Tan Kofli, and Mohd. Sahaid Kalil, (1999) Effect of pH and agitation on growth of bacillus subtilis. Sains Malaysiana, 28 . pp. 49-57. ISSN 0126-6039

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The effect of initial pH of medium (6.0,6.5 and 7.2) and agitation (100,200 and 300 rpm) on growth of oxygen sensitive culture of Bacillus subtilis were studied using shake flask cultures. Two type of media used were complex medium (CM) and well defined medium (WDM3). The effect of both parameters and types of media on production of acetoin and 2,3-butanediol were also studied. The maximum growth was detected in experiment with agitation at 300 rpm and pH 6.5 for both CM (2.87 g/l dry weight) and WDM3 (3.33 g/l dry weight). The use of WDM3 gave higher total products (acetoin+butanediol) concentration (0.67 g/l) compare to CM (0.44 g/l)

Item Type:Article
Journal:Sains Malaysiana
ID Code:3766
Deposited By: Mr Fazli Nafiah -
Deposited On:19 Mar 2012 04:46
Last Modified:02 May 2012 09:55

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