Challenges in culturing macaca fascicularisBone marrow stem cells

Sharen, S@Sharen Aini and Masfueh, R and Badiah, B and Ng, M.H and Shamsul, B.S and Chua, K.H and Low, C.K and Mohd Nazimi, A.J and Rifqah, N and Norziha, Y and Shariffah Shuriana, A.S and Ruszymah, B.H.I and Siar, C and Ramli, R (2012) Challenges in culturing macaca fascicularisBone marrow stem cells. Sains Malaysiana, 41 (4). pp. 419-422. ISSN 0126-6039


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Culturing Macaca fascicularis bone marrow stem cells in fetal bovine serum (FBS) resulted in low proliferation and long period of incubation. Therefore, its potential uses are exhausted. Here we report the establishment of culturing the Macaca fascicularis bone marrow stem cells using the FBS in combination with autologous serum. Five percent autologous serum was added to the Minimum Essential Medium (MEM) alpha medium and 10% FBS while 0.2 mM acid ascorbic 2-phosphate, 10 mM β-glycerolphosphate, 10-8 molar dexamethasone were used for osteogenic induction. Following this combination, our results showed higher growth kinetic i.e. 1.41% growth rate higher compared to only 0.46% growth rates of the cells using FBS alone and shorter population doubling time (4 to 7 days) compared to the culture without the combination of FBS and autologous serum (30 days). Thus, the combination of the FBS and autologous serum permits fast cell growth and tissue construction.

Item Type:Article
Keywords:Bone marrow stem cells; Macaca fascicularis; tissue engineering
Journal:Sains Malaysiana
ID Code:3930
Deposited By: Mr Azam
Deposited On:26 Mar 2012 08:58
Last Modified:14 Dec 2016 06:35

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