Jamilah Syafawati Yaacob, and Azani Saleh, and Hashimah Elias, and Sakinah Abdullah, and Noraini Mahmad, and Normadiha Mohamed, (2014) In vitro regeneration and acclimatization protocols of selected ornamental plants (agapanthus praecox, justicia betonica and celosia cristata). Sains Malaysiana, 43 (5). pp. 715-722. ISSN 0126-6039
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Official URL: http://www.ukm.my/jsm/
Abstract
This paper discussed on the effectiveness of BAP and NAA growth hormones on establishment of plant regeneration for selected ornamentals; Agapanthus praecox, Justicia betonica and Celosia cristata. Various explants (leaf, stem, shoot tip and bulb) derived from one-month-old aseptic seedlings of A. praecox and C. cristata, as well as explants from intact plants of J. betonica were utilized to achieve complete plant regeneration of these species. MS medium supplemented with various hormones, with an emphasis on BAP and NAA was tested to obtain direct and indirect regeneration. Both A. praecox (bulbs) and C. cristata (shoots) formed complete plantlets on MS added with 0.5-2.0 mg/L BAP and NAA, while direct regeneration was achieved for J. betonica on MS media containing BAP. Several methods were attempted to acclimatize the regenerants, with A. praecox gave the highest percentage of survival rates (96.67%), followed by J. betonica (80.00%) and C. cristata (75.00%).
Item Type: | Article |
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Keywords: | Acclimatization; Agapanthus praecox; Celosia cristata; Justicia betonica; plant growth regulators |
Journal: | Sains Malaysiana |
ID Code: | 7152 |
Deposited By: | ms aida - |
Deposited On: | 19 May 2014 06:00 |
Last Modified: | 14 Dec 2016 06:43 |
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