Isolation and identification of Aphanomyces species from natural water bodies and fish farms in Selangor, Malaysia

Afzali, S.F. and Hassan, M.D. and Abdul-Rahim, A.M. and Sharifpour, I. and Sabri, J. (2013) Isolation and identification of Aphanomyces species from natural water bodies and fish farms in Selangor, Malaysia. Malaysian Applied Biology, 42 (2). pp. 21-31. ISSN 0126-8643

[img]
Preview
PDF
1MB

Official URL: http://mabjournal.com/

Abstract

This study was conducted to isolate and identify fresh water fungi species from the Malaysian natural water bodies and fish farms and to examine the pathogenicity of the isolates as a confirmative identification tool for epizootic ulcerative syndrome (EUS) outbreak in Selangor state, Malaysia. For this aim, 165 water samples and 62 infected fish collected from 12 stations were tested in which 35 and 24 samples were found to be positive for fungi contamination and/or infection, respectively. The isolates were morphologically characterized; from 59 isolates, 32 were identified as Saprolegina, 21 as Achlya and 6 as Aphanomyces species. Experimental infection was carried out by intramuscularly injection of the Aphanomyces spp. isolates to the Malaysian moonlight gourami (Trichogaster Microlepis), where no mortality and no signs of EUS were observed in the fish groups. Histopathology test also revealed no signs of damage in the skin, muscles and other tissues following infection with the isolates indicating that all the Aphanomyces isolates were non-pathogenic.

Item Type:Article
Keywords:Aphanomyces, Saprolegnia, Achlya, Epizootic Ulcerative Syndrome (EUS), histopathology
Journal:Malaysian Applied Biology Journal
ID Code:7273
Deposited By: ms aida -
Deposited On:13 Jul 2014 05:27
Last Modified:14 Dec 2016 06:43

Repository Staff Only: item control page