Epitope mapping of Anti-YB-1 antibodies using truncated proteins

Wei, T.C. and Shamsuddin, S. (2014) Epitope mapping of Anti-YB-1 antibodies using truncated proteins. Malaysian Applied Biology, 43 (1). pp. 21-30. ISSN 0126-8643


Official URL: http://mabjournal.com/


Increased expression and nuclear localization of Y-box-binding protein-1 (YB-1) are associated with drug resistance and tumor progression. Detection of YB-1 primarily depends on the recognition of YB-1 antibody on its epitopes. To determine the epitopes binding of anti-YB-1 A-16 and 59-Q antibodies, three YB-1 truncated proteins were generated using bacterial expression system and their aberrant migration were reported. Epitope mapping analyses revealed anti-YB-1 A-16 antibody recognized epitope at 1-127 amino acid residues of YB-1, whereas 59-Q antibody was reactive with epitope at 61-127 amino acid residues. Based on our findings, 59-Q antibody was suggested used in the future study to avoid ambiguity.

Item Type:Article
Keywords:epitope mapping; YB-1; truncated protein; antibodies
Journal:Malaysian Applied Biology Journal
ID Code:7285
Deposited By: ms aida -
Deposited On:15 Jul 2014 09:00
Last Modified:14 Dec 2016 06:43

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