Molecular characterisation and expression analysis of cathepsin D from the Asian seabass lates calcarifer

Shariza Azizan, and Kiew, Lian Wan and Adura Mohd-Adnan, (2014) Molecular characterisation and expression analysis of cathepsin D from the Asian seabass lates calcarifer. Sains Malaysiana, 43 (8). pp. 1139-1148. ISSN 0126-6039


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The lysosomal aspartic proteinase cathepsin D is an acute phase protein involved in various physiological processes, including vitellogenesis, yolk processing and immune responses. In this study, we characterised the cathepsin D from the Asian seabass Lates calcarifer and examined its expression profile during infection. The complete coding sequence of L. calcarifer cathepsin D consists of 1191 nucleotides, encoding a 396 amino acid protein molecule that is made up of a putative signal peptide, a leader peptide and a mature peptide. Phylogenetic analyses showed that two types of cathepsin D are present in the teleost lineage i.e. cathepsin D1 and D2, whereas higher vertebrates possess only one type of cathepsin D. L. calcarifer cathepsin D was clustered together with cathepsin D1 from other teleosts. Compared to mammalian sequences, L. calcarifer cathepsin D lacks the β-hairpin loop that forms the double chain and is present as a single chain peptide with conserved aspartic active sites like other fish. Both multiple sequence alignment and phylogenetic analysis indicated that the L. calcarifer cathepsin D sequence codes for cathepsin D1 and suggested that it shares the same functions with cathepsin D from other fish. Expression profiling analysis of cathepsin D in L. calcarifer infected with Aeromonas hydrophila showed that it is up-regulated in immune-related tissues such as gills, spleen and liver, suggesting that cathepsin D plays an important role in the innate immune response of L. calcarifer against pathogens.

Item Type:Article
Keywords:Acute phase protein; aspartic proteinase; expression profile; innate immune response
Journal:Sains Malaysiana
ID Code:7509
Deposited By: ms aida -
Deposited On:10 Sep 2014 08:29
Last Modified:14 Dec 2016 06:44

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