Proximate analysis and anti-proliferative properties of vitex negundo L.

Abstract

The study of the proximate analysis on the leaves of Vitex negundo L. (VN) was done to gain the preliminary data of the anti-proliferative properties on cancer cell lines. Aqueous and organic extracts of the leaves of VN were used to identify its cytotoxic effect on six types of cancer-origin and normal cells, namely hormone-dependent breast cancer cell line (MCF-7), non-hormone-dependent breast cancer cell line (MDA-MB-231), ovarian cancer cell line (Caov-3), cervical cancer cell line (HeLa), liver cancer cell line (HepG2) and human foreskin fibroblast cell line (Hs27). The anti-proliferation activities of these extracts were investigated by employing colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)2,5 diphenyltetrazolium bromide) assay through time periods of 24, 48 and 72 h. Preliminary results showed that the methanol extracts had significant effects (p<0.05) on MDA-MB-231 with IC50 values 65.38 μg/mL. The nutritional composition of the leaves provides a strong basis for emphasizing the nutritional value of Vitex negundo L.