Proximate analysis and anti-proliferative properties of vitex negundo L.

Rabeta Mohd Salleh, and Neda Ganjali Dashti, and Ong, Ming Thong (2014) Proximate analysis and anti-proliferative properties of vitex negundo L. Sains Malaysiana, 43 (10). pp. 1543-1547. ISSN 0126-6039


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The study of the proximate analysis on the leaves of Vitex negundo L. (VN) was done to gain the preliminary data of the anti-proliferative properties on cancer cell lines. Aqueous and organic extracts of the leaves of VN were used to identify its cytotoxic effect on six types of cancer-origin and normal cells, namely hormone-dependent breast cancer cell line (MCF-7), non-hormone-dependent breast cancer cell line (MDA-MB-231), ovarian cancer cell line (Caov-3), cervical cancer cell line (HeLa), liver cancer cell line (HepG2) and human foreskin fibroblast cell line (Hs27). The anti-proliferation activities of these extracts were investigated by employing colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)2,5 diphenyltetrazolium bromide) assay through time periods of 24, 48 and 72 h. Preliminary results showed that the methanol extracts had significant effects (p<0.05) on MDA-MB-231 with IC50 values 65.38 μg/mL. The nutritional composition of the leaves provides a strong basis for emphasizing the nutritional value of Vitex negundo L.

Item Type:Article
Keywords:Cancer cell line; MTT assay; proximate analysis; Vitex negundo L.
Journal:Sains Malaysiana
ID Code:7817
Deposited By: ms aida -
Deposited On:04 Nov 2014 17:32
Last Modified:14 Dec 2016 06:45

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