Cyanide degradation by pseudomonas pseudoalcaligenes strain W2 Isolated from mining effluent

Belinda Tiong, and Zaratulnur Mohd Bahari, and Nor Sahslin Irwan Shah Lee, and Jafariah Jaafar, and Zaharah Ibrahim, and Shafinaz Shahir, (2015) Cyanide degradation by pseudomonas pseudoalcaligenes strain W2 Isolated from mining effluent. Sains Malaysiana, 44 (2). pp. 233-238. ISSN 0126-6039


Official URL:


Cyanide is highly toxic to the living organisms as it inhibits respiration system in the cell mitochondria. Cyanide is commonly used in gold extraction process and its discharge into the environment not only causes pollution but it also brings harm to the surrounding population. Chemical treatment is expensive and the use of hazardous compound can exacerbate the problem. Biodegradation offers cheap and safe alternative as it overcomes the problems faced by chemical treatment. In this study, indigenous bacteria from mining wastewater were isolated. Cyanide degradation was done via shake flask method. A bacterium, designated W2 was found able to grow in the mining wastewater. 16S rRNA analysis identified the strain as Pseudomonas pseudoalcaligenes which could tolerate up to 39 mg/L cyanide concentration and growth was depleted at 52 mg/L. 60% cyanide degradation was achieved in wastewater containing medium. End-product analysis from high performance liquid chromatography (HPLC) detected formamide implicating the role of cyanide hydratase in cyanide degradation. It can be concluded that P. pseudoalcaligenes is capable of biodegrading cyanide and its potential in wastewater treatment containing cyanide is feasible.

Item Type:Article
Keywords:Biodegradation; bioremediation; cyanide; mining wastewater; Pseudomonas pseudoalcaligenes
Journal:Sains Malaysiana
ID Code:8307
Deposited By: ms aida -
Deposited On:05 Mar 2015 10:36
Last Modified:14 Dec 2016 06:46

Repository Staff Only: item control page