Genome mining for glycoside hydrolases from the psychrophilic yeast Glaciozyma antarctica PI12

Nooraisyah, M.N. and Siti Nur Hasanah, M.Y. and Mahadi, N.M. and Abu Bakar, F.D. and Murad, A.M.A. (2015) Genome mining for glycoside hydrolases from the psychrophilic yeast Glaciozyma antarctica PI12. Malaysian Applied Biology, 44 (1). pp. 167-172. ISSN 0126-8643


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Glycoside hydrolases are enzymes that hydrolyse glycosidic bonds in carbohydrate chains to produce simple molecules. In fungi, glycoside hydrolases are important enzymes that hydrolyse complex carbohydrates into simple sugars that can subsequently be consumed for energy metabolism. Glaciozyma antarctica is a psychrophilic yeast isolated from sea ice in Antarctica. The G. antarctica genome has been completely sequenced, and a total of 7,857 genes have been predicted. The objective of the present study was to determine different classes of glycoside hydrolases from the G. antarctica genome and predict the localisation of these enzymes. Using genome mining, a total of 97 G. antarctica genes were predicted to encode glycoside hydrolases. The majority of the enzymes, including endoglucanases, xylanases, and chitinases, were identified from GH family 5 (12 genes), followed by GH family 45 (11 genes), GH family 10 (11 genes) and GH family 18 (9 genes). The secreted glycoside hydrolase enzymes were primarily endoglucanases from GH family 45, and these enzymes degrade celluloses in the cell walls of plants and algae. Extracellular glycoside hydrolases have been implicated as important in nutrient scavenging and organic decomposition in Antarctic sea ice.

Item Type:Article
Keywords:Glycoside hydrolases, psychrophiles, Glaciozyma antarctica, genome mining
Journal:Malaysian Applied Biology Journal
ID Code:8725
Deposited By: ms aida -
Deposited On:10 Jun 2015 00:21
Last Modified:14 Dec 2016 06:48

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