Molecular characterization of Malaysian marine fish species using partial sequence of Mitochondrial DNA 12S and 16S rRNA Markers

Siti Farah Quraishia, and Sundararajulu Panneerchelvam, and Zafarina Zainuddin, and Nur Haslindawaty Abd Rashid, (2015) Molecular characterization of Malaysian marine fish species using partial sequence of Mitochondrial DNA 12S and 16S rRNA Markers. Sains Malaysiana, 44 (4). pp. 1119-1123. ISSN 0126-6039

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Official URL: http://www.ukm.my/jsm/english_journals/vol44num8_2...

Abstract

This study was conducted to characterize the selected marine fish species using partial sequence of mtDNA 12S and 16S rRNA gene. PCR amplification of 12S and 16S rRNA generated PCR amplicons at 350 and 440 bp lengths, respectively. Sequence analysis was performed using BioEdit software. Phylogenetic tree was constructed using MEGA software. Two reference species have been used namely Gobio gobio (AB239596 and EF112528) and Pentapodus caninus (DQ533268 and DQ532933). Based on the result obtained, mtDNA 12S and 16S rRNA were found to be useful as molecular markers for fish species identification. These markers will provide correct identification of fish species when considered along with morphological characteristics.

Item Type:Article
Keywords:Mitochondrial DNA; species identification; 12S rRNA; 16S rRNA
Journal:Sains Malaysiana
ID Code:9040
Deposited By: ms aida -
Deposited On:04 Oct 2015 10:34
Last Modified:14 Dec 2016 06:48

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