The establishment of a primary colon carcinoma cell line

Zubaidah Zakaria, and Gregory A.R.A., and Tong. S, and Boon. C.F, and Asril Yusof, and Norhanom Abdul Wahab, and Latifah Saiful Yazan, and Muhammad Saifulamman Mohamed Said, (2003) The establishment of a primary colon carcinoma cell line. Jurnal Sains Kesihatan Malaysia, 1 . pp. 35-43. ISSN 1675-8161

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A primary human colon carcinoma cell line (IMR22) was established in the Institute for Medical Research from a 71 years old Chinese m,an who had a right Hemicolectomy done for a fungating mass in the carcum. The tumour mass was then confirmed histopathologically to be a well differentiated Adenocarcinoma Dukes Stage II, Apiece of the tumour tissue resected fresh from the operating theatre was grown to extablish the cell line. The initial growth medium was in Amniomax C 100 supplemented with Fungizone, Penicillin and Streplomycin and was later changed to Medium 199 with Earl’s salt supplemented with 20% fetal calf serum, Kanamycin, Penicillin and Streptomycin and Lgtutamine. The IMR22 line established after ten passages with the occurrence of single type of cell in the culture whose morphological characteristics resembled that of the primary tumour.

Item Type:Article
Keywords:Human colon carcinoma cell line;tissue culture; histophatology; growth medium; primary tumour.
Journal:Jurnal Sains Kesihatan Malaysia
ID Code:945
Deposited By: Mr Azhari Jamaludin
Deposited On:28 Apr 2011 02:14
Last Modified:14 Dec 2016 06:28

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