Assessment of DNA strand breakage in streptozotocin-induced diabetic Rats

Siti Balkis Budin, and Norfadilah Rejab, and Abdul Gapor Mohd Top, and Wan Nozaimoon Wan Mohamud, and Mokhtar Abu Bakar, and Khairul Osman, and Jamaludin Mohamed, (2005) Assessment of DNA strand breakage in streptozotocin-induced diabetic Rats. Jurnal Sains Kesihatan Malaysia, 3 (1). pp. 1-12. ISSN 1675-8161

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This study was conducted to evaluate the oxidative damage in diabetic mellitus induced rats. The evaluation of DNA damage was carried out by the Alkaline Comet Assay using peripheral lymphocyte cells taken from streptozotocin-induced diabetic rats (50 mg/kg) and control rats. The levels of malondealdehyde (MDA), 4-hydroxynonenal (4-HNE), fasting blood glucose (FBG) and HbA1c were also measured. All the induced diabetic rats were hyperglycemic until the end of the study with significantly higher levels of FBG and HbA1c as compared to the control rats. The results showed the percentage of tail DNA and tail moment values were also significantly higher in the diabetic induced rats. The same observations were made on the levels of plasma MDA and 4-HNE. In conclusion, this study indicated that hyperglycemic condition in diabetic induced rats could generate oxidative DNA damage.

Item Type:Article
Keywords:diabetes mellitus; hyperglycemia; oxidative stress; DNA damage; Alkaline Comet Assay
Journal:Jurnal Sains Kesihatan Malaysia
ID Code:982
Deposited By: Mr Azhari Jamaludin
Deposited On:29 Apr 2011 07:45
Last Modified:14 Dec 2016 06:28

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