Phylogenetic relationships within the Genus Holothuria inferred from 16S Mitochondiral rRNA Gene Sequences

Kamarul Rahim Kamarudin, and Aisyah Mohamed Rehan, and Ridzwan Hashim, and Gires Usup, and Maryam Mohamed Rehan, (2016) Phylogenetic relationships within the Genus Holothuria inferred from 16S Mitochondiral rRNA Gene Sequences. Sains Malaysiana, 45 (7). pp. 1079-1087. ISSN 0126-6039


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This study aimed to resolve the taxonomic status of a morphologically undetermined sea cucumber species of order Apodida from Malaysia (GenBank accession no.: FJ223867) using partial 16S mitochondrial rRNA gene sequences and subsequently to determine the validity of morphological taxonomy of Holothuria species into its current subgenera. The undetermined species clustered with all taxa of Holothuria in previous study. Phylogenetic analyses using maximum parsimony and Bayesian methods suggest that the undetermined species was genetically closer to Holothuria (Lessonothuria) pardalis and Holothuria (Acanthotrapeza) coluber; and its position in both phylogenetic trees further suggests its status as a Holothuria taxon. Subgenera of Holothuria, Merthensiothuria and Metriatyla are monophyletic with strong bootstrap supports and posterior probabilities of clades, thus strengthening their morphological taxonomies. Nonetheless, the non-monophyly of subgenera of Halodeima, Microthele and Platyperona suggests a requirement for their taxonomic revisions using integrative taxonomy. The status of Holothuria (Halodeima) edulis subgroups in the maximum parsimony and Bayesian trees is indistinct and further taxonomic revisions are necessary. In terms of sister relationship, both phylogenetic trees suggest that subgenus Holothuria is a sister taxon of subgenus Roweothuria while the other sister relationships were unclear due to the undetermined species, paraphyly and polyphyly of a number of subgenera. Further studies with more specimens of genus Holothuria from broader geographical locations and various mtDNA genes along with morphological approaches may facilitate to provide better insights into the molecular phylogeny of subgenera of Holothuria.

Item Type:Article
Keywords:Bayesian inference; Holothuria; Partial sequences of 16S mitochondrial ribosomal RNA gene; Phylogenetic relationship
Journal:Sains Malaysiana
ID Code:9982
Deposited By: ms aida -
Deposited On:17 Jan 2017 01:29
Last Modified:19 Jan 2017 09:30

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